ABSTRACT
The difficulty of a reliable diagnosis of pancreatic diseases by scintiscanning, is mainly derived from the lack of adequate radiopharmaceuticals. With this purpose (125) I-L(-3) Iodo-a-Methyl Tyrosine ((125) I-IMT) has been studied, which has also been used for the diagnosis of different kind of brain tumors. The purpose of this work is the development of a quick and easy method for the synthesis and purification of the (125) I-IMT in order to be used in a Nuclear Medicine Service. The L-(-Methly Tyrosine was labeled with (125) I using I-/I03 and afterwards purified by an aniomic exchange resin. The labeling yield obtained was (96.0+0.5) per cent when the incubation time was 15 minutes. No significant statistical differences were observed when the incubation time was extended to 1 hour. Biodistribution studies in mice show that the percentage of activity concentration in pancreas is (34.24+14.03) per cent at 15 minutes post injection, remaining constant for 30 minutes. The pancreas/liver ratio 15 minutes after the injection of the labeled product was (12.22+3.59) and it remained constant for 45 minutes more. These results show that (125) I-IMT cab be used as a diagnostic agent for pancreatic diseases. Since (123) I was not avilable at the moment, this new methodology was developed with (125) I.
Subject(s)
Mice , Animals , Diagnostic Imaging , Methyltyrosines , Pancreatic Neoplasms , Analysis of Variance , Electrophoresis, Paper , Iodine RadioisotopesABSTRACT
Se ajustó a la técnica de preparación del ACS.Tc-99m descrita por Dupuy y col., encontrándose el pH óptimo para su estabilidad. Se estudió la distribución biológica en ratones, determinando el porcentaje de dosis inyectada en distintos órganos, a diferentes tiempos
Subject(s)
Animals , Mice , Chondroitin Sulfates , Diagnostic Techniques, Radioisotope , Technetium , Cartilage , Drug Stability , Gamma Cameras , Tissue DistributionSubject(s)
Humans , Radionuclide Generators/instrumentation , Isotope Labeling , Radiobiology , Indium Radioisotopes , Radioisotopes , Radionuclide Imaging/standards , Sodium Pertechnetate Tc 99m/chemical synthesis , Technetium Tc 99m Pentetate/chemical synthesis , Technetium Tc 99m Sulfur Colloid/chemical synthesis , Radiobiology/instrumentation , Radiobiology/standards , Radionuclide Imaging/instrumentationABSTRACT
La benzoilmercaptoacetiltriglicina (MAG,) se sintetizó de acuerdo con el método de A.R.Fritzberg y colaboradores. Se comprobó su pureza por punto de fusión, composición centesimal, cromatografía líquida de alta presión y resonancia magnética nuclear. Se realizaron estudios farmacológicos, esterilidad, apirogenicidad, toxicidad aguda y distribución biológica en animales. El producto (MAG,) marcado con tecnecio 99m, según la técnica de J.R.Coveney y M.S.Roblins, se controló por cromatografía en papel. Posteriormente, se inyectó en seres humanos (2,5-3 ,Ci) en normales y con patología renal (39 casos totales) realizándose el estudio comparativo con hipurán